| GeneLibs

题目:: Comparative genomic hybridization of 17 Borrelia burgdorferi isolates of varying pathogenicity and RST gene type to investigate genome plasticity
ID:
状态:: 发布时间Aug. 15, 2006 , 更新时间 May 2, 2014 , 提交时间 June 15, 2006,
物种:: Borrelia burgdorferi
摘要:: B. burgdorferi isolates differ in pathogenicity, and a correlation between RST type and pathogenicity was observed: the most invasive of the isolates are associated with RST1 and the least invasive with RST3 (Wang et al. 2001, Wang et al. 2002, Wormser et al. 1999). Comparative genome hybridization employing custom B. burgdorferi 70mer oligonucleotide microarrays representing all reference strain B31MI ORFs was used to assess genome plasticity, to reveal genes that are conserved and thus may encode essential functions and to reveal elements encoding potential virulence factors in 15 B. burgdorferi isolates (B479, B515, BL206, 297, B376, B379, BL224, B331, B348, B356, B408, B418, B500, JD1, N40) representing all three RST types. Total genomic DNA for each isolate was labeled with cy3 and hybridized simultaneously with cy5-labeled B31MI DNA as reference. Each hybridization was performed in duplicate.
实验种类:: comparative genomic hybridization by array
样本量:: 17
实验设计:
: 无设计数据
数据号:: E-MEXP-735
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息