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题目:: Transcription profiling of Strongyloides ratti larval stage 2 (L2) direct vs indirect development
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状态:: 发布时间Jan. 1, 2005 , 更新时间 May 2, 2014 , 提交时间 May 3, 2006,
物种:: Strongyloides ratti
摘要:: S. ratti Isofemale line ED321 Heterogonic predominantly undergoes indirect development; isofemale line ED5 Homogonic predominantly undergoes direct development. Therefore, L2 stages of ED321 Heterogonic and of ED5 Homogonic are destined for indirect (i.e. L2 indirect) and direct (i.e. L2 direct) development, respectively; these sources of material were used in this comparison. To do this, for both isofemale lines, rats were infected with ED321 Heterogonic or ED5 Homogonic and faeces collected on days 5, 6, 7 and 8 p.i. and cultured for 24 h at 19oC, after which larvae were prepared with a Baermann funnel held for 6 h at 19oC, larvae were concentrated and cleaned by sucrose flotation, as above. In excess of 75,000 worms were routinely isolated from three infected hosts. The experimental design used, was to have at least three biological replicates for each sample (i.e. three independent preparations of the relevant worm samples and their RNA) and to have at least three technical replicates (i.e. independent, separate cDNA synthesis, amplification and hybridization etc.) for each biological replicate. For each hybridisation a dye-swap was used i.e. each sample to be used in a hybridisation was labelled, separately, with each of the two dyes.
实验种类:: transcription profiling by array
样本量:: 10
实验设计:
: 无设计数据
数据号:: E-MEXP-709
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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