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题目:: MicroRNA profiling by array of mouse lung from individuals sensitised with chicken egg OVA and then challenged with aerosolised OVA
ID:
状态:: 发布时间June 10, 2011 , 更新时间 April 27, 2011
物种:: Mus musculus
摘要:: specific pathogen-free female BALB/c mice aged 7-8 weeks (Animal Resources Centre, Perth, Western Australia) were systemically sensitised by intraperitoneal injection of 50 µg of alum-precipitated chicken egg OVA (Grade V, ?98% pure, Sigma Australia) 21 and 7 days before inhalational challenge, then exposed to aerosolised OVA in a whole body inhalation exposure chamber (Unifab Corporation, Kalamazoo, MI). Chronic low-level challenge involved exposure to ?3 mg/m3 aerosolised OVA for 30 minutes/day on 3 days/week for up to 6 weeks. Particle concentration within the chamber was continuously monitored using a DustTrak 8520 instrument (TSI, St Paul, MN). All experimental procedures complied with the requirements of the Animal Care and Ethics Committee of the University of New South Wales (reference numbers: 06/119B and 08/09B). Mice were sacrificed after 1,2,4 and 6 weeks of OVA exposure. Control groups included naïve mice and mice that were not sensitised but were challenged for 6 weeks with aerosolised OVA.
实验种类:: microRNA profiling by array
样本量:: 22
实验设计:
: 无设计数据
数据号:: E-MEXP-3118
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