实验库 数据相关信息
- 题目:
- Transcription profiling by array of human A2780 ovarianc cancer cells and cisplatin resistant derivate cells treated with cisplatin or liposomal cisplatin
- ID:
- 状态:
- 发布时间Oct. 4, 2012 , 更新时间 May 2, 2014 , 提交时间 July 30, 2010,
- 物种:
- Homo sapiens
- 摘要:
- A2780 ovarian cancer cells and a cisplatin resistant derivate of A2780 cells, obtained from ECACC, UK, No. 93112519 [A2780] and No. 93112517 [A2780 cis] were seeded out in T-75 flasks at a density of 2x106 for A2780sens and 3x106 for A2780cis cells in 15 ml of medium and preincubated overnight. Medium was removed and 15 ml fresh medium (37°C) with different concentrations of cisplatin, liposomal cisplatin or empty liposomes were added and incubated for 72 h at 37°C in a 5% CO2 incubator. In case of A2780sens cells, 1.72 µM cisplatin (IC50 concentration) and in case of A2780cis cells 8.94 µM cisplatin (IC50 concentration) were added. Liposomal formulations contained equal cisplatin concentrations. Empty liposomes were added in the same concentration as the liposomal cisplatin, to analyze the impact of liposomal lipids (A2780sens: 0.80µmol lipid, A2780cis: 4.15 µmol lipid). After incubation, medium was removed and cells were washed thrice with 10 ml PBS. 1 ml RLT-buffer was added and cells lysates were stored at -80°C until RNA extraction.
- 实验种类:
- transcription profiling by array
- 样本量:
- 8
- 实验设计:
- 无设计数据
- 数据号:
- E-MEXP-2840
- 数据状态:
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DataTable pData
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