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题目:: Transcription profiling by array of Burkholderia lata strains 383 and 383-CMIT treated with preservatives
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状态:: 发布时间May 10, 2011 , 更新时间 May 2, 2014 , 提交时间 July 20, 2010,
物种:: Burkholderia lata
摘要:: 25 ml of a modified basal salts medium (BSM; Hareland et al. 1975, J Bacteriol 121:272) supplemented with 0.05% casamino acids (Becton & Dickinson, Sparks, USA), 0.05 % yeast extract (Oxoid, Basingstoke, UK) and 0.4 % glucose (Fisher Scientific, Loughborough, UK) flasks were inoculated with 2 x 10^8 CFU and incubated at 30°C on an orbital shaker at 150 rpm. Growth was monitored spectrophotometrically. Burkholderia lata strains evaluated were: strain 383 (LMG22485) and strain 383-CMIT (a spontaneous mutant with increased tolerance to a commercially available blend of 3:1 methylisothiazolinone & chloromethylisothiazolinone preservatives). Sub-inhibitory concentrations of preservatives added at the time of inoculation were: 0.003% dimethylol dimethyl hydantoin (DDH, Lonza Ltd, Basal, Switzerland) or 0.01 % methylisothiazolinone/chloromethylisothiazolinone (MIT/CMIT, Romm & Haas, Coventry, UK). Only B. lata strain 383 was cultured with dimethylol dimethyl hydantoin. Strain 383 cultivated without preservatives was used as control condition.
实验种类:: transcription profiling by array
样本量:: 16
实验设计:
: 无设计数据
数据号:: E-MEXP-2827
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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