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题目:: Transcription profiling by array of human NKX2.1GFP/w spin embryoid bodies differentiated and then FACS sorted based on FORSE-1, NKX2.1-GFP and PDGFR expression
ID:
状态:: 发布时间Jan. 5, 2011 , 更新时间 June 3, 2014 , 提交时间 July 17, 2010,
物种:: Homo sapiens
摘要:: The gene targeted NKX2.1GFP/w hESC line was differentiated as spin EBs in BPEL medium supplemented with FGF2 and retinoic acid to generate NKX2.1-GFP+ neural cells. NKX2.1-GFP+ cells were FACS sorted and further differentiated in serum free BEL medium supplemented with FGF2 (20 ng/ml) and EGF1 (20 ng/ml) on laminin-coated flasks. After ~ 90 days in culture, cells were FACS sorted based on FORSE-1 and NKX2.1-GFP expression, with the FORSE-1-GFP- fraction further fractionated on the basis of PDGFR? expression. Sorted fractions were subjected to Illumina microarray processing.
实验种类:: transcription profiling by array
样本量:: 5
实验设计:
: 无设计数据
数据号:: E-MEXP-2808
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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