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题目:: Transcription profiling by array of human NKX2.1GFP/w spin embryoid bodies treated with all-trans retinoic acid
ID:
状态:: 发布时间Jan. 5, 2011 , 更新时间 June 3, 2014 , 提交时间 July 16, 2010,
物种:: Homo sapiens
摘要:: The gene targeted NKX2.1GFP/w hESC line was differentiated as spin EBs in BPEL medium supplemented with FGF2 (50-100 ng/ml). At day 7 of the differentiation, the spin EBs were pulsed with all-trans retinoic acid (10-5 M) for 72 hours. At day 10, spin EBs were removed from retinoic acid containing media and transferred from suspension to adherent culture in BEL medium containing no growth factors. Day 23 EBs were FACS sorted based on E-CADHERIN and NKX2.1-GFP expression. Day 0 hESCs, day 7 EBs, day 10 EBs with or without RA treatment and day 23 unsorted EBs and sorted fractions were subjected to Illumina microarray processing.
实验种类:: transcription profiling by array
样本量:: 9
实验设计:
: 无设计数据
数据号:: E-MEXP-2807
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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