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题目:: RNAi profiling by array of mouse P19 cells to investigate how SR proteins coordinate gene expression programs
ID:
状态:: 发布时间July 20, 2010 , 更新时间 May 2, 2014 , 提交时间 March 23, 2010,
物种:: Mus musculus
摘要:: Members of the SR protein family of RNA binding proteins play numerous roles in gene expression, including the regulation of pre-mRNA splicing, mRNA export, and translation. How SR proteins coordinate gene expression programs has been largely unexplored, and comprehensive knowledge of endogenous mRNA targets is lacking. Gene expression changes upon SRp20 or SRp75 RNAi in P19 parental, SRp75-BAC, and SRp20-BAC cells were measured with mouse whole genome microarrays. The knockdown of either SRp20 or SRp75 led to up- or downregulation of specific transcripts. Phenotypic rescue in the SRp75-BAC, and SRp20-BAC cells demonstrated functionality of the GFP-tagged SR proteins, and showed the specificity of the knockdowns.
实验种类:: transcription profiling by array
样本量:: 21
实验设计:
: 无设计数据
数据号:: E-MEXP-2640
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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