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题目:: Transcriptional profiling of Saccharomyces cerevisiae to identify beneficial mutations in strain BY4741 that allow growth at increased pH
ID:
状态:: 发布时间Jan. 6, 2010 , 更新时间 June 10, 2011 , 提交时间 Dec. 28, 2009,
物种:: Saccharomyces cerevisiae
摘要:: The Low MP laboratory strain BY4741 was subjected to a stepwise selection procedure during 120-150 generations, enabling enrichment for beneficial mutations that allow growth at increasing pH. In order to identify the mutations responsible for the ability to grow at high pH, we used tiling arrays to genotype the Low MP ancestor and three evolved strains: two clones from two independent lines that had acquired the ability to grow at pH 8.5 and pH 8.6 (A8.5 and C8.6) and one clone from an intermediate stage of line C (C8.0). The identity and nature of the mutated alleles was further confirmed by direct DNA sequencing.
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
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数据号:: E-MEXP-2520
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