Transcription profiling of human U2OS cell line transfected with IZ-1 and 14 ARF expressing plasmids
ID:
状态:
发布时间Nov. 1, 2009
, 更新时间 May 2, 2014
, 提交时间 Aug. 17, 2009,
物种:
Homo sapiens
摘要:
U2OS cells were stably transfected with an ecotropic receptor expression plasmid. These cells were infected with retroviruses expressing MIZ-1 or MYC and subsequently superinfected with retroviruses expressing p14ARF. Selection was carried out 48h following superinfection and selected cells were harvested within 1 - 2 passages. For each condition around 2.5x10<superscript6> cells were pooled.