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题目:: Transcription profiling of human HEL cells treated with either 5 uM of JI1 or 10 uM of Erlotinib reveal growth of leukemic Jak2-V617F positive cells is mutually suppressed by Jak inhibitors and Erlotinib through distinct mechanisms
ID:
状态:: 发布时间Aug. 10, 2009 , 更新时间 May 2, 2014 , 提交时间 July 8, 2009,
物种:: Homo sapiens
摘要:: Gene expression profiling experiments were performed using the Agilent 44K whole human genome microarrays (Agilent Biotechnologies, Diegem, Belgium) according to the two-color gene expression analysis (Quick Amp labeling) protocol (version 5.7) from the manufacturer. Hybridizations were performed by comparing 300 ng of total RNA extracted from HEL cells treated with either 5 ?M of JI1 or 10 ?M of Erlotinib with that obtained from untreated cells. For each treatment, three biological replicates were analyzed in duplicate including a dye swap. Only high quality RNAs with a ribosomal RNA ratio greater than 1.9 and no evidence of degradation, as evaluated using the Agilent Bioanalyzer 2100 RNA 6000 nano assay, were used in this study. Microarray images were quantified using the GenePix Pro 6.1 software (Molecular Devices, Sunnyvale, CA, USA) as described in supplemental information.
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-MEXP-2244
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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