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题目:: Transcription profiling of Listeria monocytogenes after infection into murine macrophages
ID:
状态:: 发布时间Nov. 25, 2010 , 更新时间 May 2, 2014 , 提交时间 Dec. 15, 2008,
物种:: Listeria monocytogenes
摘要:: P388D1 murine macrophages were cultured in 85 mm tissue culture plates to about semi confluency. L. monocytogenes serotypes (1/2a EGD-e, 4a L99, 4b CLIP80459 and 4b F2365) were infected to the P388D1 cell monolayer at a MOI of 100 per eukaryotic cell. Infection was carried for 45 min and followed by addition of fresh medium containing 20 µg/ml gentamicin. The medium of the plates (containing 20 µg/ml gentamicin) infected with L. monocytogenes serotypes were replaced after 2 h post infection with fresh medium containing 50 µg/ml gentamicin. At each step the plates were washed extensively with 1x PBS. Incubation of the bacterial tissue culture plates was carried out in a humidified incubator for up to 4 h post infection.
实验种类:: transcription profiling by array
样本量:: 55
实验设计:
: 无设计数据
数据号:: E-MEXP-1947
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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