siRNAi knockdown of ZBTB4 in human SH-EP cells reveals Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation
ID:
状态:
发布时间July 1, 2008
, 更新时间 May 2, 2014
, 提交时间 April 1, 2008,
物种:
Homo sapiens
摘要:
SH-EP cells were depleted for ZBTB4; Transfection of the cells were performed by oligofectamin RNAimax (Invitrogen) with siRNA smart pool (Dharmacon RNA Technologies); total RNA was isolated using RNAeasy Mini Kit (QIAGEN), preamplified, labeled with Cy3 and Cy5, respectively, and hybridized to cDNA-microarrays