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题目:: Transcription profiling of wild type and Foxc-1 knock-out mouse embryos
ID:
状态:: 发布时间Dec. 14, 2007 , 更新时间 May 2, 2014 , 提交时间 Dec. 12, 2007,
物种:: Mus musculus
摘要:: Wild-type vs. mutant on candidate cDNA array: Total RNA was extracted from the whole heads of 13.5 wild-type and mutant embryos using TriPure reagent (Roche) and purified using TriPure reagent was purified using the RNeasy Mini kit (Qiagen). Labelled cDNA was generated from RNA extracted from six pooled wild-type and six pooled mutant samples. Labelled cDNA was synthesised using the 3DNA Array 50 kit (Genisphere) and hybridised to the candidate cDNA array. For the oligo array, labelled cDNA was generated from RNA extracted from six pooled wild type and six pooled mutant samples. Labelled cDNA was generated using the amino-alyll labeling method.
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-MEXP-1385
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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