Transcription profiling of bark from Mexican Lime inoculated with two different isolates of the Citrus Tristeza Virus
ID:
状态:
发布时间May 18, 2007
, 更新时间 May 2, 2014
, 提交时间 May 10, 2007,
物种:
Citrus aurantifolia
摘要:
The transcriptional response of the Mexican Lime to two different isolates of the Citrus Tristeza Virus was evaluated. Virus Isolates were T305, which provokes severe symptoms in Lime plants, and isolate T385 which does not result in any visible symptoms. Five Lime plants were inoculated with each virus isolate, and four healthy plants were used to create a pooled control. Citrus custom arrays were hybridized with an infected sample and the pooled control, using a dyeswap design. In total, the experiment comprised hybridizations with twenty microarrays, ten for each virus isolate, including five biological replicates, each technically duplicated by dye-swapping.