实验库 数据相关信息

题目:
DEPS-1 promotes P granule assembly and RNA interference in C. elegans germ cells
ID:
状态:
发布时间May 26, 2010 , 更新时间 May 2, 2014 , 提交时间 Dec. 20, 2007,
物种:
Caenorhabditis elegans
摘要:
P granules are germ-cell-specific cytoplasmic structures containing RNA and protein and required for proper germ cell development in C. elegans. A new P-granule-associated protein, DEPS-1, whose loss disrupts P granule structure and function was analysed in this study. Genome wide analysis of gene expression in deps-1 mutant germ lines identified additional targets of DEPS-1 regulation, many of which are also regulated by the RNAi factor RDE-3. Our studies suggest that DEPS-1 is a key component of the P granule assembly pathway and that its roles include promoting accumulation of some mRNAs, such as glh-1 and rde-4, and reducing accumulation of other mRNAs, perhaps by collaborating with RDE-3 to generate endogenous short interfering RNAs (endo-siRNAs). We isolated dissected gonads from deps-1 mutant adults and compared each to wild type dissected gonads. RNA was linearly amplified prior to labeling for all genotypes. The comparisons were done in quadruplicate on independently grown and isolated animals.
实验种类:
unknown experiment type
样本量:
8
实验设计:
无设计数据
数据号:
E-GEOD-9993, GSE9993
数据状态:

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