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题目:: Transcription profiling of rat retina after IONT and IONC - time series
ID:
状态:: 发布时间Oct. 20, 2008 , 更新时间 Aug. 1, 2011 , 提交时间 Dec. 17, 2007,
物种:: Rattus norvegicus
摘要:: retinal ganglion cells die after optic nerve injury, either crush or transection. The molecular causesunderlying this degeneration are largely unkwon; the purpose of this job is to find which (if any) gene regulation triggers RGC death with the final goal of design neuroprotective protocols Experiment Overall Design: 3 groups: naive, IONT (intraorbital nerve transection) IONC (intraorbital nerve crush). IONT and IONC lesioned animals were kept the appropriate times postlesion (12h,. 24h, 48h, 3d, 7d, and 15d). For each time point 8-12 animals were used. RNA from 4 animals was pooled and extracted to hybridaze 1 array replica. All replicas were pooled biological replicas: 5 for naive RNA (each replica 4 retinas, therefore 5 independent RNA extractions were done with a total of 20 retinas), IONT: 12h 3 replicas, 24h 2 replicas, 48h 3replicas, 3d 2 replicas, 7d 3 replicas, 15d 2 replicas. IONC: 3 replicas per time point: 12h, 24h, 48h, 3d and 7d)
实验种类:: transcription profiling by array
样本量:: 35
实验设计:
: 无设计数据
数据号:: E-GEOD-9918, GSE9918
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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