实验库 数据相关信息
- 题目:
- Transcription profiling of human cells transfected with the recombinant adenovirus bearing no cDNA (CMV) or cDNA for ERalpha 203/204/211E (3411E) for 48h
- ID:
- 状态:
- 发布时间June 16, 2008 , 更新时间 June 10, 2011 , 提交时间 Dec. 3, 2007,
- 物种:
- Homo sapiens
- 摘要:
- In addition to the estrogen responsive element (ERE)-dependent gene expression, E2-ERalpha regulates transcription through functional interactions with transfactors bound to their cognate regulatory elements on DNA, hence the ERE-independent signaling pathway. However, the relative importance of the ERE-independent pathway in E2-ERalpha signaling is unclear. Our studies in infected ER-negative cell models with an ERalpha mutant (ERalpha 203/204/211E) that functions exclusively at the ERE-independent pathway demonstrated that genomic responses assessed by microarrays from the ERE-independent pathway to E2-ERalpha are not sufficient to alter cellular growth, death or motility. These findings suggest that the ERE-dependent pathway is the canonical E2-ERalpha signaling in model cell lines. Experiment Overall Design: Cells were infected with the recombinant adenovirus bearing no cDNA (CMV) or cDNA for ERalpha 203/204/211E (3411E) for 48h. Infected cells were then treated with 1 nM Estradiol 17beta for 6h. All experiments were repeated six independent times conducted at at six different days
- 实验种类:
- transcription profiling by array
- 样本量:
- 12
- 实验设计:
- 无设计数据
- 数据号:
- E-GEOD-9758, GSE9758
- 数据状态:
无法自动分析，您可以尝试手动分析数据。
DataTable pData
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