| GeneLibs

题目:: Transcription profiling of C. elegans slr-2, C2H2 Zn-finger protein mutant to normal controls at L1 stage
ID:
状态:: 发布时间June 16, 2008 , 更新时间 June 10, 2011 , 提交时间 Oct. 5, 2007,
物种:: Caenorhabditis elegans
摘要:: Our slr-2 dataset showed strong overrepresentation of genes previously identified in a serial analysis of gene expression (SAGE) intestinal library (McGhee et al., 2006) (p << 0.01); 812 genes were common to both data sets. Consistent with the deregulation of intestinal genes, we observed repression of several important metabolic pathways, including the TOR and insulin signaling networks, suggesting that slr-2(ku297) mutants experience metabolic stress. We also compared differentially regulated genes in slr-2 and lin-35 single mutants. Again, we saw a statistically significant overlap (p-value < 0.01); 261 genes were present in both data sets. Strikingly, > 75% of genes common both datasets showed expression changes in the same direction, although the common dataset contained an approximately equal mixture of up and downregulated genes. Furthermore, more than fifty genes common to the lin-35 and slr-2 datasets are known to have intestinal-associated functions. That some of these common intestinal genes were absent from the gut SAGE library could be due to differences in the developmental stage of the animals assayed (adults versus L1s) as well as experimental approaches (SAGE versus microarrays) Experiment Overall Design: We compared transcriptome profiles of slr-2 mutants and control N2 worms at L1 stage stage. Experiment Overall Design: Each comparison was done in triplicate with independently grown and isolated animals. Experiment Overall Design: Data for N2 L1 stage were from our previous experiments (Series GSE6547) Experiment Overall Design: To achieve higher accuracy, RMA values were calculated on the basis of 21 microarray experiments (including data from Series GSE6547)
实验种类:: transcription profiling by array
样本量:: 6
实验设计:
: 无设计数据
数据号:: E-GEOD-9246, GSE9246
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息

DataTable pData

联系方式

微信公众号

实验库 数据相关信息

DataTable pData

联系方式

微信公众号

实验库数据相关信息