Identification of EMT related genes in mcf10 cell line
ID:
状态:
发布时间May 25, 2010
, 更新时间 May 2, 2014
, 提交时间 July 9, 2007,
物种:
Homo sapiens
摘要:
In vitro studies on immortalized breast MCF10A cells were additionally performed to assess the phenotypic and genetic changes associated to mesenchymal transformation of breast cells and to their epithelial plasticity. To further study the molecular events associated to the cell plasticity of MCF10A cells, we decided to characterize the genetic programs modulated during the phenotypic changes by cDNA microarrays analysis. Cells were grown to 10-20% (sparse) or 80-100% confluence (confluent), and the corresponding RNAs were extracted and hybridized onto cDNA microarrays.