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题目:: Isolation and transcriptional profiling of embryonic human neural crest cells
ID:
状态:: 发布时间Aug. 7, 2008 , 更新时间 March 27, 2012 , 提交时间 July 2, 2007,
物种:: Homo sapiens
摘要:: To identify new candidate genes for neurocristopathies, a Long-SAGE library has been made from human neural crest cells (hNCC) derived from a normal embryo at Carnegie stage 13. Data analysis indicates the expression of a number of genes in multiple GO functional classes that were anticipated based on animal studies and/or human disease. An average linkage hierarchical clustering was performed using SAGE data from hNCC and 14 other normal tissues and cell lines. This and other analyses indicate a high degree of molecular identity between hNCC and the two human embryonic stem cells (hES) lines included, compared to the other tissue or cell types included, including more tissue-restricted stem cells, such as mesenchymal stem cells, or hNCC derivatives such as Schwann cells. Keywords: Cell type comparison Total RNA was extracted from a confluent 10 cm dish of hNCC (line N5) from a female human embryo at 28-32 dpf (Carnegie stage 13). Half the RNA was used to construct a SAGE bank with long tags using NlaIII and MmeI. Data was then compared to currently publicly available SAGE data from fourteen banks made from normal human tissues for hierarchical clustering. Third-party GEO accession numbers for human SAGE data: GSM41360, hES3; GSM41362, hES4; GSM48250, sciatic nerve; GSM48251, Schwann cells (in vitro); GSM31931, brain substantia nigra; GSM824, muscle; GSM785, liver; GSM708, kidney; GSM676, brain white matter; GSM761, brain cerebellum; GSM762, lung. Other third-party data: Cancer Genome Anatomy Project
实验种类:: transcription profiling by SAGE
样本量:: 1
实验设计:
: 无设计数据
数据号:: E-GEOD-8368, GSE8368
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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