Transcription profiling of human HEK293 cells overexpressing HNF4a2 wild type or mutant forms C106R or R154X
ID:
状态:
发布时间Oct. 18, 2008
, 更新时间 May 2, 2014
, 提交时间 June 26, 2007,
物种:
Homo sapiens
摘要:
Identification of genes regulated by the transcription factor HNF4a2 Experiment Overall Design: We used microarrays to identify genes regulated by the transcription factor HNF4a2. HEK293 cell lines containing doxycyclin-inducible wilt type or mutant forms of HNF4a2m which were introduced by FRT/FLP mediated recombination into FLP-In T-REx 293 cells (Invitrogen) were treated for 24 hours with 1 µg/ml doxycyclin. Gene expression profiles of induced and noninduced cells were compared to identify HNF4 regulated targets.