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题目:: Transcription profiling of human U937 leukemia cells in apoptosis induced by ansimycin
ID:
状态:: 发布时间June 16, 2008 , 更新时间 March 27, 2012 , 提交时间 June 21, 2007,
物种:: Homo sapiens
摘要:: Anisomycin is known as a potent apoptosis inducer by activating JNK/SAPK and inhibiting protein synthesis during translation. However, only few details are known on the mechanism of apoptosis induced by this compound. Genes in apoptosis induced by anisomycin in human leukemia U937 cells were investigated by using an Affymetrix GeneChip system. DNA fragmentation and phosphatidylserine externalization assays clearly demonstrated that anisomycin induced apoptosis in a time- and concentration- dependent manner. Of 22,283 probe sets analyzed, this compound down-regulated 524 probe sets and up-regulated 523 by a factor 1.5 or greater. Experiment Overall Design: U937 cells, a human lymphoma cell line, were incubated with anisomycin (1 μM) for 0, 1, 2, 3, and 6 h at 37°C. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with Human Expression Array U133A which was spotted with 22,283 probe sets. Sample preparation for array hybridization was carried out as described in the manufacture’s instructions.
实验种类:: transcription profiling by array
样本量:: 5
实验设计:
: 无设计数据
数据号:: E-GEOD-8229, GSE8229
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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