Transcription profiling of Medicago truncatula root meristem cells
ID:
状态:
发布时间June 16, 2008
, 更新时间 May 2, 2014
, 提交时间 June 13, 2007,
物种:
Medicago truncatula
摘要:
The root apical meristems (RAM) is established during embryogenesis and serves as a source of stem cells for plant growth and organogenesis. Young roots have a simple structure and meristeamtic and non-meristematic cells can be differentiated based on their location. We have used the Affymetrix Medicago Genome Array GeneChip to compare tissue from the root meristem and non-meristematic tissue to identify meristem specific candidate genes based on changes in gene expression before and after differentiation Experiment Overall Design: Roots were sectioned separating meristematic (root-tip) and non-meristematic root tissue; total RNA was extracted and used for hybridization to Affymetrix arrays