| GeneLibs

题目:: Transcription profiling of human CD34 cDNA over-expressing human CD34+ cells
ID:
状态:: 发布时间June 18, 2008 , 更新时间 June 10, 2011 , 提交时间 June 4, 2007,
物种:: Homo sapiens
摘要:: In order to investigate the role of CD34 antigen in haematopoietic commitment, we overexpressed the human CD34 cDNA in human CD34+ cells by retroviral gene transfer. Experiment Overall Design: In a set of 10 independent experiments, gene transfer efficiency, assessed by flow cytometry analysis of ΔLNGFR positivity, ranged 15 to 30%. Transduced cells were than purified for NGFR expression at day 3 of liquid culture with the retroviral vector. Phenotypic analysis, performed on transduced/NGFR purified cells evidenced that the CD34 transgene was highly expressed at the protein level (80% of LCD34IΔN vs 30% of controls at 5 days post-infection) up to 14 days of liquid culture.
实验种类:: transcription profiling by array
样本量:: 3
实验设计:
: 无设计数据
数据号:: E-GEOD-8003, GSE8003
数据状态:: 无法自动分析，您可以尝试手动分析数据。

实验库数据相关信息