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题目:: Transcription profiling of rat REF52 cells expressing SV40 T antigens
ID:
状态:: 发布时间Sept. 12, 2009 , 更新时间 May 2, 2014 , 提交时间 May 17, 2007,
物种:: Rattus norvegicus
摘要:: SV40 transforms cells through the action of two oncoproteins, large T antigen and small t antigen. Small t antigen targets phosphatase PP2A, while large T antigen stimulates cell proliferation and survival by action on multiple proteins, including the tumor suppressors Rb and p53. Large T antigen also binds components of the transcription initiation complex and several transcription factors. We examine global gene expression in SV40-transformed REF52 cells. SV40 transformation alters the expression of approximately 480 cellular genes. Much of this regulation is consistent with T antigen action on the Rb-E2F pathway. Experiment Overall Design: We used the Affymetrix RGU34A microarrays to probe the global mRNA expression from two wild-type REF52neo and three REF52neoT stable cell lines. Cells were grown to two days post-confluence and harvested.
实验种类:: transcription profiling by array
样本量:: 5
实验设计:
: 无设计数据
数据号:: E-GEOD-7836, GSE7836
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