主页实验库实验详情

E-GEOD-72468 SRP063070, GSE72468 ChIP-seq, other, RNA-seq of coding RNA Homo sapiens

EGFR Mutation Promotes Glioblastoma Through Epigenome and Transcription Factor Network Remodeling

·发布 2015年9月14日 ·更新 2015年9月20日

样本数

实验数

实验描述

Epidermal Growth Factor Receptor (EGFR) gene amplification and mutations are the most common oncogenic events in Glioblastoma (GBM), but the mechanisms by which they promote aggressive tumor growth are not well understood. Here, through integrated epigenome and transcriptome analyses of cell lines, genotyped clinical samples and TCGA data, we show that EGFR mutations remodel the activated enhancer landscape of GBM, promoting tumorigenesis through a SOX9 and FOXG1-dependent transcriptional regulatory network in vitro and in vivo. The most common EGFR mutation, EGFRvIII, sensitizes GBM cells to the BET-bromodomain inhibitor JQ1 in a SOX9, FOXG1-dependent manner. These results identify the role of transcriptional/epigenetic remodeling in EGFR-dependent pathogenesis and suggest a mechanistic basis for epigenetic therapy. ChIP-Seq for H3K27ac, H3K4me1, and H3K4me3, and RNA-seq for Glioblastoma (GBM) cells and/or tissues with or without EGFRvIII mutation.

样本属性

cell line

Gliobmastoma cell line

organism

Homo sapiens

organism part

dorsolateral prefrontal cortex, Gliobmastoma biopsy, hippocampus

实验信息

登记号

E-GEOD-72468

GEO 编号

SRP063070, GSE72468

实验类型

ChIP-seq, other, RNA-seq of coding RNA

物种

Homo sapiens

发布日期

2015年9月14日

更新日期

2015年9月20日

提交者

Wei Zhang、 Huijun Yang、 Timonthy C Gahman、 Shiro Ikegami、 Bing Ren、 Feng Liu、 Ciro Zanca、 Webster K Cavenee、 Gary C Hon、 Samantha Kuan、 Timothy F Cloughesy、 Zhen Ye、 Ah Y Lee、 Andrew K Shiau、 Frank B Furnari、 Paul Mischel、 Genaro R Villa、 Bowen Wei、 Greg Lucey、 Paul S Mischel、 David Jenkins、 Kristen M Turner、 Bin Li、 William H Yong、 Cathy L Barr

外部链接

ArrayExpress 原始页 NCBI GEO：SRP063070, GSE72468

分析服务

EGFR Mutation Promotes Glioblastoma Through Epigenome and Transcription Factor Network Remodeling

联系地址

微信公众号