Transcription profiling of human cells transfected with MLV and HIV1 virus-like particles
ID:
状态:
发布时间June 15, 2008
, 更新时间 March 27, 2012
, 提交时间 March 6, 2007,
物种:
Homo sapiens
摘要:
We hypothesize that cellular mRNAs are incorporated nonselectively into retrovirus particles Experiment Overall Design: This study utilizes microarray analysis to test these hypotheses. Transfected cells were grown, RNA was isolated and used for global gene expression profiling (Affymetrix Human U133 Plus 2.0 array). Statistically significant gene expression was determined as a minimum 6 counts of 9 pairwise comparisons, minimum 1.5-fold change, and p < 0.05. Further, Absolute | FC - FC SEM | >= 1.5.