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题目:: TL2_14028s_M9+arabinose_(3_passages)_LibM9_vs_Lib0_Exp.2
ID:
状态:: 发布时间June 20, 2010 , 更新时间 May 2, 2014 , 提交时间 Feb. 16, 2007,
物种:: Salmonella enterica subsp. enterica serovar Typhimurium
摘要:: Salmonella typhimurium 14028s Transposon library recovered after three consecutive rounds of growth to late log phase in M9 minimal medium (arabinose 0.4%) at 37°C with aeration, compared to an expansion of the initial library selected on Luria agar plates + kanamycin (50ug/ml), O/N at 37°C Keywords: Transposon tag analysis 2 biological replicates analysed after arbitrarily primed sampling (Klenow, DOPR degenerate primer) + specific PCR amplification (Taq DNA Pol) + T7 in vitro transcription (AmpliScribe T7) + RT labeling (Cy3) + hybridization, compared to identically processed standard library (Cy5)
实验种类:: other
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-GEOD-7058, GSE7058
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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