Transcribed ultraconserved region 339 promotes carcinogenesis by sequestering tumor suppressor microRNAs

·发布 2015年7月7日 ·更新 2015年8月20日

样本数

实验数

芯片平台

实验描述

The transcribed ultraconserved regions (T-UCRs) are a family of long non-coding RNAs implicated in human carcinogenesis. The mechanism of action of T-UCRs and the factors regulating their expression in human cancers are poorly understood. In this study we show that high expression of uc.339 correlates with lower survival in 204 non-small cell lung cancer (NSCLC) patients. We also show that uc.339 found up-regulated in archival NSCLC samples, functions as a decoy for miR-339-3p, -663-3p and -95-5p. As a result, Cyclin E2, a direct target of these microRNAs is up-regulated, promoting cancer growth and migration. We provide evidence from cell lines and primary samples suggesting that TP53 directly regulates uc.339. Our results support a key role for uc.339 in lung cancer. 4 experimental groups in triplicate (total: 12 samples). A339= A549 infected with a lentivirus over-expressing uc.339; AE= A549 infected with an empty lentivirus (control of A339); L339= LoVo infected with a lentivirus over-expressing uc.330; LE= LoVo infected with an empty lentivirus (control of L339). 1-2-3 refer to each experiment.

芯片平台

A-AFFY-44

Affymetrix GeneChip Human Genome U133 Plus 2.0 [HG-U133_Plus_2]（12 例）

样本属性

cell line

A549, LoVo

histology

adenocarcinoma

organism

Homo sapiens

实验信息

登记号

E-GEOD-70540

GEO 编号

GSE70540

实验类型

transcription profiling by array

物种

Homo sapiens

发布日期

2015年7月7日

更新日期

2015年8月20日

提交者

Kishore B Challagundla、 Erika Bandini、 Silvia Carloni、 Cristina Ivan、 Cecilia Fernandez-Cymering、 Zhihi Guo、 Ite A Laird-Offringa、 Han Liang、 Giorgia Paliaga、 Hui Ling、 Meropi Plousiou、 Xinna Zhang、 Amelia Cimmino、 Dino Amadori、 Massimo Negrini、 Petra M Wise、 Patrick Nana-Sinkam、 Barbara J Gitlitz、 Francesca Fanini、 George A Calin、 Muller Fabbri、 Manuela Ferracin、 Ramana V Davuluri、 Melissa Crawford、 Leng Han、 Maria Cortez、 Ivan Vannini、 Mariam Murtadha

外部链接

ArrayExpress 原始页 NCBI GEO：GSE70540

分析服务

Transcribed ultraconserved region 339 promotes carcinogenesis by sequestering tumor suppressor microRNAs

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