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题目:: Estimating networks from RNA interference and microarray data
ID:
状态:: 发布时间Jan. 28, 2009 , 更新时间 May 2, 2014 , 提交时间 Feb. 14, 2007,
物种:: Homo sapiens
摘要:: We here used the technology of RNA interference and cDNA microarrays to reconstruct a network between genes known to be disregulated in ER-positive breast cancer diseases. Therefor single genes of high importance were silenced and the effect of this pertubation was measured with cDNA microarrays Keywords: network_reconstruction_RNAi human ER-positive breast cancer cell line was cultivated as described and transfection with chemically synthesized siRNAs was performed according to standard HiPerFect transfection protocol by Qiagen (Hilden, Germany) At least 4 cDNA-microarrays were hybridized in dye-swap method for the silencing of every gene of interest. To minimize off-target effects we used at least two different siRNAs per gene.
实验种类:: transcription profiling by array
样本量:: 120
实验设计:
: 无设计数据
数据号:: E-GEOD-7033, GSE7033
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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