Whole genome characterisation of chemoresistant ovarian cancer [NanoString miRNA]

·发布 2015年5月19日 ·更新 2015年5月24日

121

样本数

121

实验数

芯片平台

实验描述

Patients with high-grade serous ovarian cancer (HGSC) have experienced little improvement in overall survival, and standard treatment has not advanced beyond platinum-based combination chemotherapy, during the past 30 years. To understand the drivers of clinical phenotypes better, here we use whole-genome sequencing of tumour and germline DNA samples from 92 patients with primary refractory, resistant, sensitive and matched acquired resistant disease. We show that gene breakage commonly inactivates the tumour suppressors RB1, NF1, RAD51B and PTEN in HGSC, and contributes to acquired chemotherapy resistance. CCNE1 amplification was common in primary resistant and refractory disease. We observed several molecular events associated with acquired resistance, including multiple independent reversions of germline BRCA1 or BRCA2 mutations in individual patients, loss of BRCA1 promoter methylation, an alteration in molecular subtype, and recurrent promoter fusion associated with overexpression of the drug efflux pump MDR1. Total RNA was hybridised to NanoString miRNA Human v2.1 probes, immobilized to NanoString cartridge and analysed on the NanoString Digital Analyzer. NanoString nSolver Analysis Software was utilised to check QC metrics and extract raw miRNA counts. Expression was normalised for input using the housekeeping genes. Contributor: The Australian Ovarian Cancer Study Group

芯片平台

A-GEOD-19765

NanoString nCounter miRNA Human v2.1（121 例）

样本属性

cell type

high grade serous ovarian cancer, normal

organism

Homo sapiens

organism part

ascites, fallopian tube, PrimaryTumour, Primarytumour, TumourLocalRecurrence, TumourMetastasisToDistantLocation, Tumourmetastasistodistantlocation

sex

female

实验信息

登记号

E-GEOD-65819

GEO 编号

GSE65819

实验类型

transcription profiling by array

物种

Homo sapiens

发布日期

2015年5月19日

更新日期

2015年5月24日

提交者

Greg Young、 Yoke E Chiew、 Conrad Leonard、 Charlotte Wilhelm-Benartzi、 Ed Curry、 Ehsan Nourbakhsh、 Katia Nones、 Jason Ellul、 Robert Brown、 Dale W Garsed、 Nadia Traficante、 Felicity Newell、 Michael C Quinn、 Peter J Wilson、 Paul Waring、 Scott Wood、 Ivon Harliwong、 Kate Strachan、 John V Pearson、 Patricia O'Brien、 Senel Idrisoglu、 Jan Pyman、 Nathan Hall、 Paul Harnett、 Suzanne Manning、 Jillian Hung、 Euan Stronach、 Timothy J Bruxner、 Mark Shackleton、 Timothy P Holloway、 Andrew Lonie、 Walid Azar、 Emma Markham、 Sian Fereday、 Dariush Etemadmoghadam、 Ravikiran Vedururu、 Matthew Anderson、 Michael Quinn、 Gisela Mir Arnau、 Sean M Grimmond、 Collin Stewart、 Jodie Leditschke、 Maria A Doyle、 Richard W Tothill、 Orla McNally、 Ernst Lengyel、 Darrin F Taylor、 J L Fink、 Joshy George、 David D Bowtell、 Elizabeth L Christie、 Chris Mitchell、 Andrea Jewell、 Angelika N Christ、 Nick Waddell、 Hani Gabra、 Nicola Waddell、 San Leong、 David K Miller、 Anne Hamilton、 Stephen Kazakoff、 Craig Nourse、 Ann-Marie Patch、 Prue Cowin、 Barsha Poudel、 Qinying Xu、 Kathryn Alsop、 George Au-Yeung、 Timothy Semple、 Kelly Quek、 Michael Friedlander、 Catherine Kennedy、 Stephen Cordner、 Linda Mileshkin、 Huei San、 Peter J Bailey、 Shivashankar H Nagaraj、 Karin S Kassahn、 Ronny Drapkin、 Joy Hendley、 Anna deFazio、 Heather Thorne、 Oliver Holmes

外部链接

ArrayExpress 原始页 NCBI GEO：GSE65819

分析服务

Whole genome characterisation of chemoresistant ovarian cancer [NanoString miRNA]

联系地址

微信公众号