Gene expression profiling of preneoplastic K8-creERT2/PIK3CAH1047R mammary subsets

·发布 2015年8月12日 ·更新 2016年10月18日

样本数

实验数

芯片平台

相关文献

实验描述

This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from K8-creERT2/PIK3CA H1047R mice Mammary cell subpopulations were isolated from K8-creERT2/PIK3CAH1047R and K8-creERT2 control animals 4 weeks after activation of PIK3CA H1047R transgene expression by Tamoxifen injection. Pooled mammary glands of 2-3 estrus-synchronized mice per genotype were sorted in 3 independent sortings and used for microarray analysis (20 samples in total).

参考文献

PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours

Koren S, Reavie L, do Couto JP, De Silva D, Stadler MB, Roloff T, Britschgi A, Eichlisberger T, Kohler H, Aina O, Cardiff RD, Bentires-Alj M

PMID: 26266975

芯片平台

A-AFFY-130

Affymetrix GeneChip Mouse Gene 1.0 ST Array [MoGene-1_0-st-v1]（20 例）

样本属性

cell type mammary cell subpopulation

basal CD24low Sca1 negative subset, control, basal H1047R-expressing subset, basal subset without PIK3CA H1047R-expressing cells, luminal CD24high Sca1 negative H1047R-expressing subset, luminal CD24high Sca1 negative subset, control, luminal CD24high Sca1 positive H1047R-expressing subset, luminal CD24high Sca1 positive subset, control

genotype/variation animal

K8-creERT2 control, K8-creERT2/PIK3CA H1047R

organism

Mus musculus

strain

mixed background FVB/CD-1

实验信息

登记号

E-GEOD-65411

GEO 编号

GSE65411

实验类型

transcription profiling by array

物种

Mus musculus

发布日期

2015年8月12日

更新日期

2016年10月18日

提交者

Stephane Thiry、 Mohamed Bentires-Alj、 Tim Roloff、 Shany Koren、 Tim Roloff

外部链接

ArrayExpress 原始页 NCBI GEO：GSE65411

分析服务

Gene expression profiling of preneoplastic K8-creERT2/PIK3CAH1047R mammary subsets

联系地址

微信公众号