实验库 数据相关信息
- 题目:
- Characterization of human malaria parasite Plasmodium falciparum eIF4E homologue and mRNA 5′ cap status
- ID:
- 状态:
- 发布时间June 10, 2010 , 更新时间 May 2, 2014 , 提交时间 Nov. 23, 2006,
- 物种:
- Plasmodium falciparum
- 摘要:
- The mRNA 5′ cap is normally essential for eukaryotic mRNA translation, stabilization and transport and both the cap and eIF4E are important elements of post-transcriptional gene regulation. To further our understanding of mRNA translation in the human malaria parasite Plasmodium falciparum, we have investigated the parasite translation initiation factor eIF4E and its interaction with 5′ capped mRNA. We have purified P. falciparum eIF4E as a recombinant protein and demonstrated that it has canonical mRNA 5′ cap binding activity. We used this protein to purify P. falciparum full-length 5′ capped mRNAs from total parasite RNA. Microarray analysis comparing total and eIF4E-purified 5′ capped mRNAs shows that a subset of 34 features were more than two-fold under-represented in the purified RNA sample, including 19 features representative of nuclear transcripts. The uncapped nuclear transcripts may represent a class of mRNAs targeted for storage and cap removal. Keywords: total RNA vs purified capped mRNA The microarray data were obtained from four hybridizations using RNA from two independent GST-PfeIF4E purifications from separate malaria cultures.
- 实验种类:
- other
- 样本量:
- 8
- 实验设计:
- 无设计数据
- 数据号:
- E-GEOD-6356, GSE6356
- 数据状态:
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DataTable pData
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