BATSeq of ESC-FCS/ESC-2i/NSC

·发布 June 9, 2015 ·更新 June 13, 2015

195

样本数

195

实验数

相关文献

实验描述

Single-cell polyadenylation site quantification 48 single cells each of murine embryonic stem cells maintained in FCS+LIF medium ("ESC"), embryonic stem cells maintained in serum free, 2i containing medium ("2i") and neural stem cells ("NSC") were sequenced by BATSeq, a single cell transcriptomic protocol for mapping polyadenylation sites in single cells.

参考文献

Single-cell polyadenylation site mapping reveals 3' isoform choice variability.

Velten L, Anders S, Pekowska A, Jï¿½rvelin AI, Huber W, Pelechano V, Steinmetz LM

PMID: 26040288

样本属性

batch barcode

AAGCTA, ACATCG, CGTGAT, GCCTAA, TACAAG, TGGTCA

cell barcode

AGCGCT, AGTCTT, ATAGAT, ATCGTG, ATTCCG, CACTGT, CATTCA, CCACTC, CGAAAC, CGTACG, CTCTAC, CTGATC, GAATGA, GAGAGT, GCTACC, GCTGTA, GGAACT, GTATAG, TCCGTC, TCGGGA, TCTGAG, TGTGCC, TTCGAA, TTGCGG

cell cycle stage

cell type

embryonic stem cell, ES-derived neural progenitor cells

growth medium

10% FBS + 10ng/mL LIF, N2 & B27, PD0325901 (1 μM), CHIR99021 (3 μM) and LIF (10 ng/ml)

organism

Mus musculus

实验信息

登记号

E-GEOD-60768

GEO 编号

GSE60768, SRP045791

实验类型

RNA-seq of coding RNA

物种

Mus musculus

发布日期

June 9, 2015

更新日期

June 13, 2015

提交者

Aino I Järvelin、 Lars Velten、 Lars Velten、 Wolfgang Huber、 Vicente J Pelechano、 Lars M Steinmetz、 Simon Anders、 Aleksandra Pekowska

外部链接

ArrayExpress 原始页 NCBI GEO：GSE60768, SRP045791

分析服务

BATSeq of ESC-FCS/ESC-2i/NSC

联系地址

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