Gene expression profiling of preneoplastic Lgr5-creERT2/PIK3CAH1047R mammary subsets

·发布 2015年8月12日 ·更新 2016年10月18日

样本数

实验数

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相关文献

实验描述

This study examined the effect of mutant PIK3CAH1047R expression in mammary subsets of preneoplastic mammary glands from Lgr5-creERT2/PIK3CA H1047R mice Mammary cell subpopulations were isolated from Lgr5-creERT2/PIK3CA H1047R and Lgr5-creERT2 control animals 4 weeks after activation of PIK3CA H1047R transgene expression by Tamoxifen injection. Pooled mammary glands of 2-3 estrus-synchronized mice per genotype were sorted in 3 independent sortings and used for microarray analysis (24 samples in total).

参考文献

PIK3CA(H1047R) induces multipotency and multi-lineage mammary tumours

Koren S, Reavie L, do Couto JP, De Silva D, Stadler MB, Roloff T, Britschgi A, Eichlisberger T, Kohler H, Aina O, Cardiff RD, Bentires-Alj M

PMID: 26266975

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A-AFFY-130

Affymetrix GeneChip Mouse Gene 1.0 ST Array [MoGene-1_0-st-v1]（24 例）

样本属性

animal

Lgr5-creERT2 control mice, Lgr5-creERT2/PIK3CA H1047R mice

cell type mammary cell subpopulation

basal subset H1047R-expressing, basal subset Lgr5-expressing, basal subset without Lgr5 and H1047R-expression, basal subset without Lgr5-expression, luminal subset CD24high Sca1 negative, luminal subset CD24high Sca1 negative H1047R-expressing, luminal subset CD24high Sca1 positive, luminal subset CD24high Sca1 positive H1047R-expressing

organism

Mus musculus

organism part

preneoplastic mammary gland

strain

mixed background FVB/C57BL6

实验信息

登记号

E-GEOD-59870

GEO 编号

GSE59870

实验类型

transcription profiling by array

物种

Mus musculus

发布日期

2015年8月12日

更新日期

2016年10月18日

提交者

Stephane Thiry、 Shany Koren、 Tim Roloff、 Mohamed Bentires-Alj

外部链接

ArrayExpress 原始页 NCBI GEO：GSE59870

分析服务

Gene expression profiling of preneoplastic Lgr5-creERT2/PIK3CAH1047R mammary subsets

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