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题目:: Transcription profiling of human HepG2 cells transfected with PGC-1 variants to identify ERRalpha dependent PGC-1alpha target genes
ID:
状态:: 发布时间June 14, 2008 , 更新时间 June 10, 2011 , 提交时间 Oct. 4, 2006,
物种:: Homo sapiens
摘要:: PGC-1 transcription factor was customized to limit its interations to ERRalpha. This mutant (2x9) was used to dissect the transcription activation patterns that are attributable to the PGC1-ERR interaction and PGC-1 actions that are independent of ERR. Inactive mutant with the deleted LLXXL motifs (L2L3) and wt PGC-1 were used as negative and positive controls respectively. BGAL-expressing construct was used to control for non-specific effects of adenoviral infection. Experiment Overall Design: Expression constructs for BGAL control and three variants of PGC-1 were introduced into HepG2 cells via adenoviral infection. RNA was isolated 24 hrs post infection and the changes in gene expression were detected using GeneChip technology (Affymetrix). Hybridization was performed on the HG-133 plus 2 platform. The experiment was performed in three biological replicates. Raw expression data were normalized using MAS5.0 algorithm.
实验种类:: transcription profiling by array
样本量:: 12
实验设计:
: 无设计数据
数据号:: E-GEOD-5968, GSE5968
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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