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题目:: Transcription profiling of human granulocytic differentiation of primary CD34+ cells cultured under 20% O2 levels
ID:
状态:: 发布时间June 14, 2008 , 更新时间 May 2, 2014 , 提交时间 Sept. 26, 2006,
物种:: Homo sapiens
摘要:: Little is known about the global transcriptional program underlying granulocytic (G) commitment, differentiation and maturation. Using DNA microarrays and Q-RT-PCR, we examined the transcriptional profile of G differentiation of human CD34-positive hematopoietic stem and progenitor cells cultured with interleukin-3, interleukin-6 granulocyte colony stimulating factor and stem cell factor. The goal this study was to identify genes involved in the various facets of G differentiation including commitment, expansion, differentiation and functional capacity. Experiment Overall Design: G-CSF mobilized peripheral blood CD34-positive cells were cultured with IL-3, IL-6, SCF and G-CSF under 20% O2. Three biological replicate experiments were analyzed and approximately 15% of the samples were technically replicated. Hybridizations were performed in a reference design with all samples labeled with Cy3 and a reference RNA pool labeled with Cy5.
实验种类:: transcription profiling by array
样本量:: 58
实验设计:
: 无设计数据
数据号:: E-GEOD-5917, GSE5917
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