实验库 数据相关信息

题目:
Transcription profiling of murine neurosphere cultures carrying a Bcl2 transgene and treated with histone deacetylase inhibitor Trichostatin A and demethylating agent AzaC to study DNA methylation and histone deacetylation in neural stem cells
ID:
状态:
发布时间Dec. 19, 2007 , 更新时间 March 27, 2012 , 提交时间 Aug. 12, 2003,
物种:
Mus musculus
摘要:
Neurosphere cultures were established from individually isolated E 14.5 forebrains of mouse embryos that carry a bcl2 transgene. Single-cell suspensions were prepared, seeded at 1x105 cells/ml and treated for two days with 150 nM Trichostatin A (TSA; histone deacetylase inhibitor), 500 nM 5-Aza-2-deoxycytidine (AzaC; demethylating agent) or both compounds, or left untreated. Two independent experiments were performed.
实验种类:
transcription profiling by array
样本量:
8
实验设计:
无设计数据
数据号:
E-GEOD-587, GDS538, GSE587
数据状态:

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