Transcription profiling of human aortic endothelial cells treated with with oligomeric procyanidins extracts
ID:
状态:
发布时间June 13, 2008
, 更新时间 June 10, 2011
, 提交时间 Aug. 17, 2006,
物种:
Homo sapiens
摘要:
Human aortic endothelial cells were grown in culture until confluent. In three experiments using cells derived from three separate donors confluent cultures were incubated for 6 h with contol medium, or medium containing either extracts of oligomeric procyanidins from cranberry juice or red wine, or a procyanidin-rich grape seed extract. At the end of the 6 h treatment period conditioned media samples were retained for immunoassay of secreted peptides and proteins, and RNA was extracted for microarray analysis. Experiment Overall Design: Each experiment used cells from one donor. Treatment conditions were: control medium, cranberry extract (CRE), grape seed extract (GSE), and red wine extract (RWE).