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题目:: Transcription profiling of mouse dorsal aorta derived mesangioblast clone comparison- U74B
ID:
状态:: 发布时间June 13, 2008 , 更新时间 March 27, 2012 , 提交时间 July 23, 2003,
物种:: Mus musculus
摘要:: Mouse dorsal aorta from E9.5 mouse embryos (C57BL6) was grown as an explant culture as described elsewhere (De Angelis et al., 1999). After 5 days the explant was dissociated into a single cell suspension, and plated at limiting dilution on a feeder layer of Mitomycin C-treated primary embryonic mouse fibroblasts, in 96 multiwell plates in complete medium, RPMI 20% Fetal Calf Serum. After 1 week, mesangioblast clones appeared approximately 2-4% of the wells. When the clones had grown to approximately 103 cells they were passed on gelatin-coated dishes, and further expanded. Clones D16 and D351 were used for microarray analysis.
实验种类:: transcription profiling by array
样本量:: 2
实验设计:
: 无设计数据
数据号:: E-GEOD-554, GSE554, GDS554
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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