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题目:: SAM PMN Parturition
ID:
状态:: 发布时间June 5, 2010 , 更新时间 March 27, 2012 , 提交时间 July 17, 2003,
物种:: Bos taurus
摘要:: Bovine blood neutrophil RNA from 4 primiparous Holstein cows was collected on days -7, 0, 0.25, and 1 (relative to parturition on day 0). RNA was pooled across the four animals at the independent time points to obtain the 20 ug necessary for use in a loop design. RNA pools were separated into two 10 ug aliquots prior to cDNA synthesis and dye coupling in a loop design. Each sample was labeled with Cy3 and Cy5 and run in paired hybridizations as follows: GSM8506 compaired day 0.25 and day -7, GSM8507 compaired day 1 and day 0, GSM8508 compaired day -7 and day 1, GSM8509 compaired day 0 and 0.25. This pairing strategy was utilized to directly compare day -7 samples with days 0.25 and 1 samples because results from previous experiments suggested that the most pronounced differences in gene expression would occur between these days. Keywords = microarray, cDNA, bovine, neutrophil, parturition
实验种类:: unknown experiment type
样本量:: 8
实验设计:
: 无设计数据
数据号:: E-GEOD-544, GSE544
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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