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题目:: Comparison of small medium and large healthy ovarian pig follicles
ID:
状态:: 发布时间June 30, 2010 , 更新时间 June 10, 2011 , 提交时间 July 12, 2006,
物种:: Sus scrofa
摘要:: Three different stages of pig antral follicles have been studied in a granulosa-cell transcriptome analysis on nylon microarrays (1152 clones). The data have been generated from 7 RNA follicle pools and several technical replicates were made. Four Large, one Medium and two Small follicles pools were considered. For each follicle pool, 2 radioactive labellings were performed. Each membrane was exposed 16 hours (to avoid saturation of the signal of highly expressed genes) and 28 hours (to get some signal from lowly expressed genes). Each probe was hybridised on GPL3971 scag_scai Sus scrofa 1.2K mono array and on GPL3970 scag_scai Sus scrofa 4.6K triplicate array (except for GFS2172 which was labelled only once and hybridised onto 2 GPL3971 scag_scai Sus scrofa 1.2K mono array membranes), so that 4 spots are available for each gene (and 2 spots for GFS2172), for a given RNA and a given radioactive labelling. Keywords: granulosa-cell transcriptome analysis data consisted in (6 RNA x 2 labellings) + (GFS2172 RNA X 1 labelling)= 13 probes, 26 hybridisations, 52 images.
实验种类:: transcription profiling by array
样本量:: 52
实验设计:
: 无设计数据
数据号:: E-GEOD-5299, GSE5299
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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