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题目:: Transcription profiling of E. coli BL21, BL21/pOri1 and BL21/pOri2mutants
ID:
状态:: 发布时间June 13, 2008 , 更新时间 June 10, 2011 , 提交时间 July 6, 2006,
物种:: Escherichia coli
摘要:: Transcription profiles in BL21, BL21/pOri1 and BL21/pOri2 were analysed using DNA microarray technology. BL21, BL21/pOri1 or BL21/pOri2 strains were cultured at chemostat status and harvested after the cultivation arrived steady status. Experiment Overall Design: BL21, BL21/pOri1 and BL21/pOri2 were cultured at the exponential growth status or at the same growth rate status, respectively. E. coli BL21, BL21/pOri1 and BL21/pOri2 RNAs were reverse-transcripted into cDNAs, The RNAs from the BL21, BL21/pOri1, BL21/pOri2 were labelled with biotin. The labelled cDNAs were mixed and then hybridized on the microarray slides. Experiments were repeated four times. Most of them have no significant difference comparing plasmid-carrying E. coli and plasmid-free E. coli
实验种类:: transcription profiling by array
样本量:: 24
实验设计:
: 无设计数据
数据号:: E-GEOD-5239, GSE5239
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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