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题目:: Functional genomics of cactus host shifts in Drosophila mojavensis
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状态:: 发布时间July 1, 2010 , 更新时间 March 27, 2012 , 提交时间 June 22, 2006,
物种:: Drosophila mojavensis
摘要:: Understanding the genetic basis of adaptation to novel environments remains one of the major challenges confronting evolutionary biologists. While newly developed genomic approaches hold considerable promise for addressing this overall question, the relevant tools have not often been available in the most ecologically interesting organisms. Our study organism, Drosophila mojavensis, is a cactophilic Sonoran Desert endemic utilizing four different cactus hosts across its geographic range. Its well-known ecology makes it an attractive system in which to study the evolution of gene expression during adaptation. As a cactophile, D. mojavensis oviposits in the necrotic tissues of cacti, therefore exposing larvae and even adults to the varied and toxic compounds of rotting cacti. We have developed a cDNA microarray of D. mojavensis to examine gene expression associated with cactus host use. Using a population from the Baja California population we examined gene expression differences of third instar larvae when reared in two chemically distinct cactus hosts, agria (Stenocereus gummosus, native host) vs. organpipe (S. thurberi, alternative host). We have observed differential gene expression associated with cactus host use in genes involved in metabolism and detoxification. The experiment was composed of 5 sets of dye-flips (rep1-5). Larvae were reared in either necrotic agria or organpipe cactus tissues. They were then collected at the third instar stage and its total RNA extracted.
实验种类:: unknown experiment type
样本量:: 20
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数据号:: E-GEOD-5148, GSE5148
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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