Transcription profiling of human CD* T lymphocytes overexpressing hTERT
ID:
状态:
发布时间June 13, 2008
, 更新时间 March 27, 2012
, 提交时间 June 23, 2006,
物种:
Homo sapiens
摘要:
Using CD8+ T lymphocyte clones over-expressing telomerase weinvestigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone. HD1 was profiled on U133A and submitted as a separate GEO series. Experiment Overall Design: triplicates of 4 conditions, 1 condition (GFP YOUNG) lost because of problems in cell culture.