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题目:: Bovine tissue gene expression profiling at peak lactation
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状态:: 发布时间July 1, 2010 , 更新时间 Oct. 18, 2011 , 提交时间 March 17, 2006,
物种:: Bos taurus
摘要:: Tissue from mammary, liver, and three adipose depots (mesenteric, omental, and subcutaneous) was collected at slaughter from a cow at peak lactation to examine gene expression profiles of 7872 sequences using a bovine cDNA microarray. RNA (20 ug) from tissues and a reference standard derived from a mixture of tissues were used to make aminoallyl-labeled cDNA followed by incorporation of Cy3-ester and Cy5-ester (Amersham, Piscataway, NJ). Each experimental sample was co-hybridized with the reference standard, which allowed the treatment of fluorescence ratios as measurements of relative expression across all samples and time points. Probes were hybridized to the array for 2 days at 42 C. All samples were hybridized to duplicate slides for a total of 4 spots per cDNA element. The microarray platform has already been described [Everts et al. (2005) Vet. Immunol. Immunopathol. 105:235-245]. Slides were scanned for both dye channels with a Scanarray 4000 (GSI-Lumonics, Billerica, MA) dual-laser confocal scanner and images were processed using GenePix 4.0 (Axon Instruments, Inc., CA).
实验种类:: unknown experiment type
样本量:: 20
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: 无设计数据
数据号:: E-GEOD-4495, GSE4495
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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