Transcription profiling of Arabidopsis thaliana response to the bacterial pathogen Pseudomonas syringae ES4326
ID:
状态:
发布时间June 13, 2008
, 更新时间 March 27, 2012
, 提交时间 March 9, 2006,
物种:
Arabidopsis thaliana
摘要:
In order to protect themselves from pathogens, plants activate a battery of defense pathways, many of which involve changes in gene expression. We are interested in identifying plant genes that are differentially expressed in response to pathogen exposure, with the ultimate goal of studying the roles of these genes in plant defense. Experiment Overall Design: Whole plants are grown in soil, and individual leaves from 3-4 week old plants are injected with a bacterial suspension, or a mock (5mM MgSO4) control. Approximately five leaves are harvested 24 hours after inoculation and used for RNA preparation, labeling and Affymetrix hybridization.