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题目:: Transcription profiling of human Burkittas lymphoma cell line, P493-6 to identify hypoxia responsive genes
ID:
状态:: 发布时间June 12, 2008 , 更新时间 March 27, 2012 , 提交时间 Jan. 23, 2006,
物种:: Homo sapiens
摘要:: Activation of glycolytic genes by HIF-1 is considered critical for metabolic adaptation to hypoxia. We found that HIF-1 also actively suppresses glucose metabolism through the tricarboxylic acid cycle (TCA) by directly trans-activating the gene encoding pyruvate dehydrogenase kinase 1 (PDK1). PDK1 inactivates the TCA cycle enzyme, pyruvate dehydrogenase (PDH), which converts pyruvate to acetyl-CoA. Forced PDK1 expression in hypoxic HIF-1α-null cells increases ATP levels, attenuates hypoxic ROS generation and rescues these cells from hypoxia-induced apoptosis. These studies reveal a novel hypoxia-induced metabolic switch that shunts glucose metabolites from the mitochondria to glycolysis to maintain ATP production and to prevent toxic ROS production. Experiment Overall Design: We sought to determine by microarray analysis of gene expression the genes responsive to hypoxia using the human B lymphocyte cell line, P493-6. Hypoxia-responsive genes were globally assessed in cells incubated in 0.1% O2 for 29 hours at which the highest HIF-1 levels were obtained
实验种类:: transcription profiling by array
样本量:: 4
实验设计:
: 无设计数据
数据号:: E-GEOD-4086, GSE4086, GDS1807
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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