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题目:: Transcription profiling of Arabidopsis Hsa32 mutant and wild type plants subjected to heat shock
ID:
状态:: 发布时间June 27, 2007 , 更新时间 March 27, 2012 , 提交时间 Jan. 18, 2006,
物种:: Arabidopsis thaliana
摘要:: Plants and animals share similar mechanisms in the heat-shock (HS) response, such as synthesis of the conserved HS proteins (Hsps). However, because plants are confined to a growing environment, in general they require unique features to cope with heat stress. We have analyzed the function of a novel Hsp, heat-stress-associated 32-kD protein (Hsa32), which is highly conserved in land plants but absent in most other organisms. The gene responds to HS at the transcriptional level in moss, Arabidopsis, and rice. Like other Hsps, Hsa32 protein accumulates greatly in Arabidopsis seedlings after HS treatment. Disruption of Hsa32 by T-DNA insertion does not affect growth and development under normal conditions. However, the acquired thermotolerance in the knockout line was compromised following a long recovery period (> 24 h) after an acclimation HS treatment, when a severe HS challenge killed the mutant but not the wild-type plants, but no significant difference was observed if they were challenged within a short recovery period. Microarray analysis of the knockout mutant indicates that only the expression of Hsa32 was significantly altered in HS response. Taken together, our results suggest that Hsa32 is not required for the induction but maintenance of acquired thermotolerance. This report provides direct evidence that a plant-specific Hsp plays an important role in thermotolerance.
实验种类:: transcription profiling by array
样本量:: 8
实验设计:
: 无设计数据
数据号:: E-GEOD-4062, GDS1982, GSE4062
数据状态:: 无法自动分析，您可以尝试手动分析数据。

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